Effectiveness of Sonication on Diminishing Bacterial Overgrowth in Agrobacterium-mediated Transformation
نویسندگان
چکیده
منابع مشابه
Genetic Transformation of Oat Mediated by Agrobacterium is enhanced with Sonication and Vacuum Infiltration
Background: Oat (Avena sativa) with high nutritive value and fiber content is used as the main food grain in many countries for human diet as well as animal feed. Recently, it became difficult to transfer new genes through the conventional breeding due to the lack of desirable traits. Objectives: The current study aimed at achieving a st...
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To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
متن کاملAgrobacterium Mediated Transformation of Maize (Zea mays L.)
Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
متن کاملA Preliminary Experiment on Agrobacterium tumefaciens-Mediated Transformation of the P5CS1 GENE in Tall Fescue
Abiotic stress conditions can be imposed on plants through either poor water quality or adverse climate condition. These types of stress affect plant growth and development throughout their life cycle. Plants that are tolerant to drought are therefore valuable, and turf grasses have the potential to grow tolerant. Turf grasses provide ground cover and offer multiple benefits, from their aesthet...
متن کاملSonication and Vacuum Infiltration Enhanced Agrobacterium Rhizogenes Mediated Transformation in Soybean
Objective: This study involved the formulation of protocol of Agrobacterium rhizogenes mediated transformation for the detailed study of isoflavones (IFs) metabolism in soybean. Methods: Cotyledons were separated from 4-day-old soybean seedlings and infected with three different A. rhizogenes strains under various time durations of sonication, vacuum infiltration and cocultivated on Murashige a...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2004
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.21.53